| 质粒名称: | pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 163056 |
| 存储实验室: | Adam Cohen |
|
载体骨架基本信息 |
|
| 载体名称: | pLX304 |
| 载体出品公司: | -- |
| 空载体大小: | 9377 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | 杀稻瘟菌素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mito-roGFP2-Grx1 |
| 物种来源: | Synthetic |
| 插入大小: | 1254 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | CMV-F |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial) was a gift from Adam Cohen (Addgene plasmid # 163056 ; http://n2t.net/addgene:163056 ; RRID:Addgene_163056) |
| 参考文献部分: | Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. 10.1038/s41467-020-17607-5 PubMed 32753572 |
pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochon
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondr... 9003 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial).
ACCESSION .
VERSION .
KEYWORDS pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondr...
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9003)
AUTHORS Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
TITLE Multiplexed Optical Sensors in Arrayed Islands of Cells for
multimodal recordings of cellular physiology.
JOURNAL Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
PUBMED 32753572
REFERENCE 2 (bases 1 to 9003)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9003
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..181
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 228..353
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 846..1079
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1264..1308
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1483..1983
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1902..1920
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1995..2390
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 2450..2567
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2684..2987
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2988..3191
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3142..3162
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 3214..3238
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3242..3316
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 3341..4054
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(3383..3404)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3644..3663)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3991..4012
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(4520..4544)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4546..4587
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 4629..5217
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4682..4702)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5100..5111)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5289..5522
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5600..5721
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5637..5656)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5691..5710
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5761..5896
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5823..5842
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5916..5935)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5917..5935)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5945..5962)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5945..5961)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5954..5976)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6103..6558
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6190..6209)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6400..6421
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6584..6688
/gene="bla"
/label=AmpR promoter
CDS 6689..7549
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6907..6926)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7720..8308
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8209..8228
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8462..8479
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8596..8617
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8632..8662
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8670..8686
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8675..8697
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8694..8710
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8694..8710
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8729..8749
/label=T3
/note="T3 promoter, forward primer"
promoter 8731..8749
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 8777..9003
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
ORIGIN
1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggctac
3241 catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc ggcggctccc
3301 agtgccgcgc gccaagatcc attcgttgcc gccgggatcc gtgagcaagg gcgaggagct
3361 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
3421 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
3481 ctccaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
3541 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
3601 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
3661 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
3721 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaactg
3781 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
3841 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
3901 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcacct gctccgccct
3961 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
4021 cgggatcact ctcggcatgg acgagctgta caagactagt ggtggttcag gtggtggtgg
4081 ttcaggtggt ggtggttcag gtggaggagg atcaggagga ggaggatcag gaggaggagg
4141 atcaggagga gaattcgctc aagagtttgt gaactgcaaa atccagcctg ggaaggtggt
4201 tgtgttcatc aagcccacct gcccgtactg caggagggcc caagagatcc tcagtcaatt
4261 gcccatcaaa caagggcttc tggaatttgt cgatatcaca gccaccaacc acactaacga
4321 gattcaagat tatttgcaac agctcacggg agcaagaacg gtgcctcgag tctttattgg
4381 taaagattgt ataggcggat gcagtgatct agtctctttg caacagagtg gggaactgct
4441 gacgcggcta aagcagattg gagctctgca gggtacctct agaggatctt tgtgaaggaa
4501 ccttacttct gtggtgtgaa cccagctttc ttgtacaaag tggttggtaa gcctatccct
4561 aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
4621 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
4681 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
4741 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
4801 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
4861 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
4921 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
4981 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
5041 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
5101 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
5161 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
5221 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
5281 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
5341 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
5401 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
5461 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
5521 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
5581 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
5641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
5701 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
5821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
5881 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
5941 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
6001 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
6061 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
6121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
6181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
6241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
6301 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
6361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
6421 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
6481 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
6541 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
6601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
6661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
6721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
6781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
6841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
6901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
6961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
7021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
7081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
7141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
7201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
7261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
7321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
7381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
7441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
7501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
7561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
7621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
7681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
7741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
7801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
7861 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
7921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
7981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
8041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
8101 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
8161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
8221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
8281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
8341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
8401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
8461 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
8521 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
8581 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
8641 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
8701 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
8761 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
8821 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
8881 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
8941 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
9001 aac
//
